Skip to main content

Cellular stress defense

Oct. 12, 2018, 10:45 AM

by Leigh MacMillan

Small heat-shock proteins (sHSPs) are molecular chaperones that bind to unfolded proteins to prevent protein aggregation and defend against cellular stress. Mutations in human sHSPs are associated with inherited diseases including cataract and cardiomyopathy.

Individual sHSPs bind to each other to form oligomers. Hassane Mchaourab, PhD, and colleagues previously discovered that insertion of a small peptide into a bacterial sHSP could cause it to expand from a 24-subunit oligomer to a 48-subunit oligomer.

The investigators wondered if flexibility in the structure of oligomers contributes to sHSP function. Now, in studies led by Sanjay Mishra, PhD, they showed that altering the sequence and length of the inserted peptide generates an array of oligomers with 30 to 38 subunits. The non-uniform collection of oligomers bound to a model protein with higher affinity.

The findings, reported in the journal Structure, support a model in which modular sHSP architecture contributes to the ability of sHSPs to serve as chaperones for a range of unfolded proteins.

This research was supported by the National Institutes of Health (grant EY012018).

Recent Stories from VUMC News and Communications Publications

Marissa Benchea has CF, and she is one of hundreds of thousands of adults not only surviving but thriving with a chronic childhood disease.

Vanderbilt Medicine

Marissa Benchea has CF, and she is one of hundreds of thousands of adults not only surviving but thriving with a chronic childhood disease.

One hundred years ago, multiple “waves” of a deadly flu swept across the world.

Vanderbilt Medicine

One hundred years ago, multiple “waves” of a deadly flu swept across the world.

A diagnosis of cancer at any age is tragic, but during the adolescent and young adult years, it’s especially complicated.

Hope

A diagnosis of cancer at any age is tragic, but during the adolescent and young adult years, it’s especially complicated.

Karen Dyer Young cares for patients and members of the Dayani Center who have or are recovering from cancer or a stem cell transplant.

Momentum

Karen Dyer Young cares for patients and members of the Dayani Center who have or are recovering from cancer or a stem cell transplant.

more