What protein is that?Mar. 28, 2018, 12:00 PM
Imaging mass spectrometry (IMS) makes it possible to see the spatial distribution of molecules in a thin tissue section based on their molecular masses. It is a powerful tool for biomarker discovery, but identifying proteins detected by IMS remains a difficult task.
The investigators automated liquid extraction surface analysis, which involves dispensing small volumes of solvent onto the tissue surface to extract proteins and other compounds, following by traditional protein-identification (proteomic) techniques.
In the March 15 Rapid Communications in Mass Spectrometry, they report robotic surface micro-extractions from thin tissue sections using a glass capillary setup, coupled directly to liquid chromatography/mass spectrometry for proteomic analysis. They found improved reproducibility and droplet resolution with the robotic extraction.
Coupling the extraction directly to the analysis is more suitable for high-throughput spatial proteomic experiments and will aid in the challenge of identifying proteins observed by IMS.
This research was supported by grants from the National Institutes of Health (OD012359, GM103391).
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