Skip to main content

The yin and yang of cell signaling

Feb. 14, 2019, 12:15 PM

by Sanjay Mishra

Lysophospholipids (LysoPLs) are potent cellular signaling biomolecules that also maintain the structure, shape and fluidity of cell membranes.

Imbalance in LysoPL levels has been associated with cell proliferation, inflammatory processes and neurological diseases.

In mammals, cellular levels of LysoPLs are finely regulated primarily by the enzymes lysophospholipase (LYPLA) 1 and 2. Although the enzymes are similar in their protein sequence and structures, they display moderate substrate specificity.

To better understand their role in LysoPL metabolism, Lawrence Marnett, PhD, and colleagues used CRISPR-Cas9 technology to generate stable knock-outs of LYPLA1 and/or LYPLA2 in mouse neuroblastoma cell lines.

Their work, published this month in the Journal of Lipid Research, showed that LYPLA1 and LYPLA2 compensated for the loss of each other and cooperatively maintained LysoPL levels in the cells.

However, deletion of both enzymes led to dramatically increased LysoPL levels and increased activation of the mitogen-activated protein kinase (MAPK) signaling pathway, which has been implicated in development of human neurodegenerative diseases and cancer.

This research was supported by grants from the National Institutes of Health (CA089450, ES007028, GM120879) and the Hancock Jr. Memorial Cancer Center Fund.

Recent Stories from VUMC News and Communications Publications

The first few minutes of Charlie’s life were a blur, as a team of doctors and nurses at VUMC worked to resuscitate him and stabilize his heart rate. He was then transferred to the Neonatal Intensive Care Unit at Monroe Carell Jr. Children’s Hospital at Vanderbilt.

Hope

The first few minutes of Charlie’s life were a blur, as a team of doctors and nurses at VUMC worked to resuscitate him and stabilize his heart rate. He was then transferred to the Neonatal Intensive Care Unit at Monroe Carell Jr. Children’s Hospital at Vanderbilt.

Tucked away in a Vanderbilt conference room, 36 adults huddle over Lego pieces. Eleven teams have been assigned to assemble multicolored Legos using the written directions included in the packet. The result should be a Frankenstein figure.

Vanderbilt Nurse

Tucked away in a Vanderbilt conference room, 36 adults huddle over Lego pieces. Eleven teams have been assigned to assemble multicolored Legos using the written directions included in the packet. The result should be a Frankenstein figure.

Marissa Benchea has CF, and she is one of hundreds of thousands of adults not only surviving but thriving with a chronic childhood disease.

Vanderbilt Medicine

Marissa Benchea has CF, and she is one of hundreds of thousands of adults not only surviving but thriving with a chronic childhood disease.

One hundred years ago, multiple “waves” of a deadly flu swept across the world.

Vanderbilt Medicine

One hundred years ago, multiple “waves” of a deadly flu swept across the world.

more